BIO/CSC295 2009F, Class 25: Time for work on projects Admin: * Welcome back! We hope you had a wonderful break and that you're ready for purgatory week. * Projects are now due next Tuesday. * We'll use class time this week to work on the projects. * You are expected to show up to class. * I believe that I've updated the schedule appropriately. * Sam remains behind in his grading. Sorry. * We'll talk more about other class work on Thursday. Overview: * Discuss in small groups * Quick Summaries at 1:30 * What your group has accomplished * How you want to use today * Discuss * Work * Think * Ask Questions Shotgun Group * Our goal is to ... build a useful shotgun sequencing algorithm and apply it to some real data * So far we have ... written an algorithm to "randomly" chop up a sequence to give ourselves test data * Today we will ... write an algorithm to simulate restriction enzymes to give ourselves more realistic test data * We need help with ... things Chou-Fasman Group * Our goal is to ... improve Chou-Fasman by focusing on trans-membrane proteins (build a new table and test the efficacy of that table) * So far we have ... found a database with information on trans-membrane proteins * Today we will ... figure out how to parse the relevant data out of the text file * We need help with ... parsing the text file Replacement matrix group * Our goal is to ... discover the evolutionary history of listeria bacteria; using BLAST to find phylogenetic data and come up with a model * So far we have ... decided what we're doing; found the important gene * Today we will ... figure out the direction for our odyssey; think about how much variability we'll allow * We need help with ... working on it MicroRNA group * Our goal is to ... Find regulatory regions controlling the expression of MicroRNA * So far we have ... found a collection of MicroRNA to study; written a program to translate format to FASTA; gotten ready to BLAST everything. * Today we will ... figure out how to get upstream sections * We need help with ... how to work with an entire genome, particularly how to figure out locations